Season 2025, Episode 42: Unlocking Cellular Diversity with spvAPA | A New Framework for Analyzing Alternative Polyadenylation in Single-Cell and Spatial Transcriptomics
In this episode of SciBud, join host Rowan as we delve into a groundbreaking study on alternative polyadenylation (APA)—a crucial cellular process that allows for the diversity of messenger RNA and, consequently, proteins. Discover the innovative analytical framework called spvAPA, specifically designed to analyze APA using single-cell and spatial transcriptomics data. We’ll break down how spvAPA enhances the detection of APA signatures, reveals hidden cellular subpopulations, and improves the understanding of gene regulation. While the study showcases impressive capabilities, we also discuss valuable critiques from experts in the field, emphasizing the importance of data transparency and the consideration of complex relationships in datasets. Tune in to learn how spvAPA may revolutionize our understanding of cellular diversity and its implications in health and disease!